Nucleosomes to Hamper the CRISPR-Cas 9 Cleavage Efficiency, Finds Researchers


A team of researchers from University of Utah has discovered that nucleosomes can hinder CRISPR-Cas 9 cleavage productivity. A paper published by the team of researchers in Proceedings of the National Academy of Science, the team talks about testing the gene editing on yeast samples. The paper consists of the final results and their findings about the test on the yeast.

The gene editing technique mentioned above utilizes RNA to locate and extract the segments of DNA. But the major question remain what happens to the targeted segment if it is a part of nucleosome? Researches which were done earlier exhibited that cleavage efficiency is likely to suffer. In this new test, the team of researchers found out that in vivo test of such instance and concluded that the result found in the previous test were absolutely correct. The use of CRISPR-Cas 9 in nucleosome is not likely to work well on nucleosome.

Nucleosome are basically rolled bunches of DNA strands around a given protein. According to logic, editing a DNA strands is a very complex process due to accessibility issues. However, other researchers have supported this logic by studying it in test tubes or have worked on known strands that were not the part of nucleosome.

The team of researchers found out that the cleavage efficiency was much less in nucleosome than in non-nucleosome areas. Apart from these, another finding in the test was that when the researchers used the gene editing technique which was called zinc fingers, even in that they did not find any dissimilarity.

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Rohit Bhisey

Rohit Bhisey

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